Composite

Part:BBa_K3015005

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-14)


pTheo-RBS

This is the transcriptional Theophylline-Riboswitch BBa_K3015004 from BOKU-Vienna fused to the Promotor BBa_J23105 on the 5' and to the RBS BBa_B0034 on the 3'.
This part can be place upstream of a gene of interest in oder to up-regulate the expression of downstream genes after induction with the target - Theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None